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植物名称:石楠(Phofinia serrulafa)又名千年红。材料类别:20年树龄的侧枝嫩芽。培养条件:以MS为基本培养基,附加不同的生长调节剂:(1)BA0.5mg/L(单位下同)+NAA0.1;(2)BA2+NAA0.3:(3)BA4+NAA1;(4)1/2MS_0(不加任何激素)。以上培养基中均加入3％蔗糖,0.6％的琼脂粉,pH5.8。培养温度25±2℃,光照度1500～2000lx,每天照光12小时。生长与分化情况: 1.外植体增殖:在石楠生长季节,取侧枝嫩芽,流水冲洗,在75％乙醇中浸20秒,无菌条件下,再用5％的安替福民灭菌15分钟,无菌水冲洗4次,切取0.5～1cm长的嫩芽接种在(1),(2),(3)培养
Plant name: Heather (Phofinia serrulafa), also known as Millennium red. Material category: 20 year old shoots. (2) BA2 + NAA0.3: (3) BA4 + NAA1 (1) BA0.5mg / L (unit same below) + NAA0.1; ; (4) 1 / 2MS_0 (without any hormones). Above medium were added 3% sucrose, 0.6% agar, pH5.8. Incubation temperature 25 ± 2 ℃, light intensity 1500 ~ 2000lx, light 12 hours a day. Growth and differentiation: 1. Proliferation of explants: In the heather growing season, take the shoots, water wash, immersed in 75% ethanol for 20 seconds, sterile conditions, and then 5% 15 minutes, rinse 4 times with sterile water, cut 0.5 ~ 1cm long shoots inoculated in (1), (2), (3) culture