论文部分内容阅读
应用经改良的外周血单个核细胞(PBMC)白细胞间介素2(IL2)产生及活性检测的方法,我们测定了不同类型乙型肝炎(乙肝)25例以及对照组11例PBMC产生的IL2活性,发现:(1) 乙肝患者产生IL2的功能低下,以重型肝炎为甚(P<0.001);(2) IL2活性的高低与抗-HBc-IgM的阴或阳性状态有关(P<0.05);(3) IL2活性下降与乙肝患者白细胞总数和淋巴细胞数量的下降之间无明显关系(r<0.3,P>0.05)。我们还动态观察了慢性迁延性肝炎患者的IL2活性及其与谷丙转氨酶活性变化之间呈负相关,并对小鼠淋巴母细胞对检测IL2活性的特异性作了实验上的论证。
Using modified interleukin 2 (IL2) production and activity assay of peripheral blood mononuclear cells (PBMCs), we measured the IL2 activity of 25 PBMCs from different types of Hepatitis B (HBV) and 11 PBMC from the control group (P <0.001); (2) The level of IL2 activity correlated with the negative or positive status of anti-HBc-IgM (P <0.05); (2) (3) There was no significant relationship between the decrease of IL2 activity and the decrease of leukocyte count and lymphocyte count in patients with hepatitis B (r <0.3, P> 0.05). We also dynamically observed IL2 activity in patients with chronic persistent hepatitis and its relationship with changes in alanine aminotransferase activity was negatively correlated, and mouse lymphoblast cells to test the specificity of IL2 activity was experimentally demonstrated.