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目的:观察止哮汤对哮喘大鼠气道重塑及肺组织增殖细胞核抗原(PCNA)的影响。方法:选用健康Wistar大鼠60只,雌雄不拘,体重(200±20)g,随机分为6组:正常对照组(A组),哮喘模型组(B组),地塞米松对照组(C组),中药低剂量组(D组)、中剂量组(E组)、高剂量组(F组)共6组,10只/组。利用卵蛋白加氢氧化铝复制哮喘大鼠模型,各组大鼠均经末次激发24 h后处死。取左肺在矢状面取材按常规方法作病理切片及HE染色。光镜下观察哮喘大鼠的气道改变;免疫组化法分别测定大鼠肺组织PCNA蛋白的表达。结果:模型组大鼠气道壁及气道平滑肌面积明显增厚,气道壁面积、气道平滑肌面积以及支气管平滑肌细胞数经支气管内周长标准化后,亦明显高于对照组,表明在哮喘急性加重期哮喘大鼠已出现了气道平滑肌的增殖和气道重建,证明了在哮喘急性期存在着气道重塑。正常对照组PCNA呈阴性表达,模型组PCNA表达呈强阳性,强于正常对照组;地塞米松组、中药各剂量组PCNA表达均弱于模型组;中药低剂量组强于地塞米松组;中药中、高剂量组则与地塞米松组无差异;中药中、高剂量组均弱于中药低剂量组;中药高剂量组与中药中剂量组表达无差异。结论:止哮汤可下调哮喘大鼠肺组织中PCNA的表达,减轻气道重塑,高、中剂量组疗效较好,与地塞米松效果相当。
Objective: To observe the effect of Zhihutang on airway remodeling and proliferating cell nuclear antigen (PCNA) in asthmatic rats. Methods: Sixty healthy Wistar rats were randomly divided into 6 groups: normal control group (group A), asthma model group (group B), dexamethasone control group (c) Group D), middle dose group (E group), high dose group (F group), 6 rats in total, 10 rats / group. Asthma rat model was made by ovalbumin plus aluminum hydroxide. Rats in each group were sacrificed 24 h after the last challenge. Take the left lung in the sagittal drawing by conventional methods for pathology and HE staining. The airway changes of asthmatic rats were observed under light microscope. The expression of PCNA protein in lung tissue of rats was detected by immunohistochemistry. Results: The area of airway wall and airway smooth muscle in model group was significantly thicker, and the area of airway wall, the area of airway smooth muscle and the number of bronchial smooth muscle cells in normal group were also significantly higher than those in control group Acute exacerbation Asthmatic rats have developed airway smooth muscle proliferation and airway remodeling, demonstrating the existence of airway remodeling in the acute phase of asthma. The expression of PCNA in normal control group was negative, and the expression of PCNA in model group was stronger than that in normal control group. The expression of PCNA in dexamethasone group and traditional Chinese medicine group was weaker than that in model group. Chinese medicine, high-dose group and dexamethasone group no difference; Chinese medicine, high-dose group were weaker than the traditional Chinese medicine low-dose group; Chinese medicine high-dose group and traditional Chinese medicine dose group no difference. Conclusion: Zhixiu decoction can down-regulate the expression of PCNA in the lung tissue of asthmatic rats and relieve airway remodeling. The high and middle dose groups have a good effect, which is equivalent to dexamethasone.