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采用浸渍叶碟饲喂法测定了坡柳皂苷对大菜粉蝶的生物活性。结果表明:用500、1 625、2 750、3 875和5 000μg/mL的坡柳皂苷处理24 h后,对大菜粉蝶4龄幼虫生长发育的抑制率分别为14.47%、31.54%、49.70%、62.48%和79.94%,处理48 h后的抑制率分别为2.72%、39.26%、54.82%、70.53%和86.39%;坡柳皂苷对大菜粉蝶4龄幼虫具有显著的拒食活性,供试5种浓度下处理24 h后,拒食率分别为30.21%、40.62%、54.33%、72.47%和84.84%,48 h后的拒食率为4.4%~86.67%;5 000μg/mL处理14 d后,幼虫化蛹率为32%,显著低于对照组(72%);供试5种浓度坡柳皂苷处理23 d后,蛹的羽化率分别为56%、44%、44%、36%和32%,均低于对照组(64%)。研究表明,坡柳皂苷对大菜粉蝶生长发育、取食及化蛹均有明显的抑制作用。
The bioactivity of Paeonia lactiflora to Pieris rapae was determined by using immersion leaf dish feeding method. The results showed that the inhibition rates of 4th and 5th instar larvae of P. tabulaeformis were 14.47%, 31.54% and 49.70%, respectively, after being treated with different concentrations of WP (500,1 625,2 750,3 875 and 5 000 μg / mL for 24 h) 62.48% and 79.94% respectively. The inhibitory rates after 48 h treatment were 2.72%, 39.26%, 54.82%, 70.53% and 86.39%, respectively. Paeoniflorin had significant antifeeding activity on the 4th instar larvae of Pieris rapae. The antifeedant rate was 30.21%, 40.62%, 54.33%, 72.47% and 84.84%, respectively. The antifeedant rate was 4.4% ~ 86.67% after 48 h treatment. After larvae were treated with 5 000 μg / mL for 14 d, The rate of emergence was 32%, which was significantly lower than that of the control group (72%). The emergence rates of pupae were 56%, 44%, 44%, 36% and 32% Lower than the control group (64%). Studies have shown that the willow saponins big cabbage butterfly growth and development, feeding and pupation were significantly inhibited.