为了解肝细胞癌（ＨＣＣ）中ｐ５３基因的突变情况，收集８６例ＨＣＣ手术切除标本，采用聚合酶链反应－单链构象多态性（ＰＣＲ／ＳＳＣＰ）、聚合酶链反应－限制性片段长度多态性（ＰＣＲ／ＲＦＬＰ）和ＤＮＡ序列分析，研究了ｐ５３基因的突变情况。８６例ＨＣＣ中，ｐ５３基因的突变率为３６％，其中，密码子２４９的突变率为３３．７％，１例ＳＳＣＰ和ＲＦＬＰ均提示密码子２４９存在突变者经ＤＮＡ序列分析显示密码子２４９的第三个碱基发生了ＡＧＧ／ＡｒｇＡＧＴ／Ｓｅｒ突变。以上结果提示，ＨＣＣ中，ｐ５３基因的突变主要以点突变为主，主要发生在密码子２４９，其突变率、突变谱和突变方式与启东等ＨＣＣ高度流行区相似 In order to understand the mutation of p53 gene in hepatocellular carcinoma (HCC), 86 cases of HCC were collected for the resection of HCC. PCR-SSCP, polymerase chain reaction-restriction fragment length Polymorphism (PCR / RFLP) and DNA sequence analysis were used to investigate the mutation of p53 gene. In 86 cases of HCC, the mutation rate of p53 gene was 36%, in which the mutation rate of codon 249 was 33.7%. One case of SSCP and RFLP both showed that there was a mutation in codon 249. DNA sequence analysis showed that codon 249 The third base has AGG / ArgAGT / Ser mutations. The above results suggest that in HCC, p53 gene mutation mainly in point mutations, mainly in codon 249, its mutation rate, mutation and mutation similar to Qidong and other HCC highly-endemic areas