近年来,酸性非特异性酯酶(ANAE)活性染色法已被广泛地应用于检测小鼠及人类外周血、脑脊液、关节滑膜液及组织切片中T淋巴细胞。但该法仍较繁琐,许多因素可直接影响染色效果。为此,我们对该法的步骤进行简化和改进,并对一些影响因素进行了探讨。 材料和方法 一、检测对象:JCR、C57BL/6J、BAIB/C小鼠及非近交系昆明种小鼠。另外检测了5名正常成年人。 二、溶液的配制:基本上参照Horwitz的方法(Horwitz D A et al:Clin Exp Immunol,30: In recent years, acid non-specific esterase (ANAE) activity staining method has been widely used to detect T-lymphocytes in mouse and human peripheral blood, cerebrospinal fluid, synovial fluid and tissue sections. However, the law is still cumbersome, many factors can directly affect the dyeing effect. To this end, we simplified and improved the steps of the law and discussed some influential factors. Materials and methods First, the test object: JCR, C57BL / 6J, BAIB / C mice and non-inbred Kunming mice. In addition, 5 normal adults were tested. Second, the solution preparation: Basically refer to Horwitz’s method (Horwitz D A et al: Clin Exp Immunol, 30: