论文部分内容阅读
对ＥＢ病毒基因在鼻咽癌活检组织细胞内的转录进行了较系统的探测。实验结果表明，ＥＢ病毒基因组在鼻咽癌活检组织中以附加体（Ｅｐｉｓｏｍｅ）形式存在，而其基因转录有如下特征：（１）ＥＢ病毒在所有鼻咽癌组织细胞中都表达ＥＢＮＡ－１，并且此基因转录产物由一个在ＢａｍＨＩ－Ｆ区的启动子（Ｆｐ）驱动；（２）；潜伏感染膜蛋白（Ｌａｔｅｎｔｍｅｍｂｒａｎｅｐｒｏｔｅｉｎ，ＬＭＰ和末端蛋白（Ｔｅｒｍｉｎａｌｐｒｏｔｅｉｎ，ＴＰ）的转录只在约５０％的活检组织中检测到，并且显示利用不同启动子的现象；（３）少部分的病例表达个别裂解基因；（４）在所有活检组织中，ＥＢ病毒基因组的ＢａｍＨＩ－Ａ区，表达一组功能未明的ｍＲＮＡ。通过对ＥＢ病毒基因在鼻咽癌细胞和其它受ＥＢ病毒感染的Ｂ淋巴细胞中表达情况的比较，本文对ＥＢ病毒基因在鼻咽癌组织细胞中的表达调控及其在癌变过程中的可能作用进行了讨论。
The transcription of Epstein-Barr virus gene in nasopharyngeal carcinoma biopsies was systematically investigated. The results showed that the Epstein-Barr virus genome exists in the nasopharyngeal biopsy tissue as an Episome, and its gene transcription has the following characteristics: (1) Epstein-Barr virus expresses EBNA-1 in all nasopharyngeal carcinoma cells, (2); transcription of latent membrane protein (LMP) and terminalprotein (TP) was only detected in about 50% of biopsy tissues (3) a small proportion of cases expressed individual lytic genes; (4) in all biopsies, the EBV genome was expressed in the BamHI-A region of a set of undefined mRNA Through the comparison of the expression of Epstein - Barr virus gene in nasopharyngeal carcinoma cells and other EB virus - infected B lymphocytes, the expression regulation of Epstein - Barr virus gene in nasopharyngeal carcinoma cells It was discussed the possible role in the carcinogenesis process.