论文部分内容阅读
目的探讨Aurora-A高表达对食管癌KYSE150细胞与细胞间黏附能力的影响。方法体外培养人食管癌KYSE150细胞,在Lipofectamine 2000介导下,分别转染高表达质粒p EGFP-C1-Aurora-A及载体p EGFP-C1,同时设空白细胞对照组(未转染)。通过Western blot法检测人食管癌KYSE150细胞中Aurora-A的表达;采用细胞缓慢聚集及细胞分离试验观察Aurora-A高表达对细胞与细胞间黏附能力的影响。结果 Aurora-A高表达组可见相对分子质量约72 000的GFP-Aurora-A融合蛋白条带,而空载体对照组及空白细胞对照组未见该蛋白条带。Aurora-A高表达组、空白细胞对照组及空载体对照组细胞团数分别为(71.0±9.4)、(25.3±0.6)和(28.5±4.0)个,N_(TC)/N_(TE)值分别为0.162±0.026、0.087±0.016和0.080±0.008,Aurora-A高表达组细胞团数及NTC/NTE值明显高于空白细胞对照组及空载体对照组(P<0.05)。结论 Aurora-A高表达能显著降低食管癌细胞与细胞间的黏附能力,进而增加肿瘤细胞的恶性表型。
Objective To investigate the effect of Aurora-A overexpression on the adhesion between KYSE150 cells and cells. Methods Human esophageal cancer KYSE150 cells were cultured in vitro and transfected with pEGFP-C1-Aurora-A and pEGFP-C1, respectively. The cells were transfected with blank control group (untransfected). The expression of Aurora-A in human esophageal cancer KYSE150 cells was detected by Western blot. The effect of Aurora-A overexpression on cell-cell adhesion was observed by slow cell aggregation and cell separation assay. Results The GFP-Aurora-A fusion protein band with a relative molecular mass of about 72,000 was observed in the high expression group of Aurora-A, but not in the empty vector control group and the blank control group. The number of cell clusters in the Aurora-A high expression group, the blank cell control group and the empty vector control group were (71.0 ± 9.4), (25.3 ± 0.6) and (28.5 ± 4.0), respectively, (0.162 ± 0.026,0.087 ± 0.016,0.080 ± 0.008, respectively). The number of cell clusters and NTC / NTE in Aurora-A overexpression group were significantly higher than those in blank control group and empty vector control group (P <0.05). Conclusion Aurora-A high expression can significantly reduce esophageal cancer cell adhesion between cells and thus increase the malignant phenotype of tumor cells.