【摘 要】
：
建立了离子对反相高效液相色谱(IP-RPHPLC)同时测定大鼠血浆和红细胞(RBC)中磷酸肌酸(PCr)及其代谢产物肌酸(Cr)以及相关三磷酸腺苷(ATP)浓度的方法.采用Kromasil-C18色谱柱(
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建立了离子对反相高效液相色谱(IP-RPHPLC)同时测定大鼠血浆和红细胞(RBC)中磷酸肌酸(PCr)及其代谢产物肌酸(Cr)以及相关三磷酸腺苷(ATP)浓度的方法.采用Kromasil-C18色谱柱(250 mm×4.6 mm,5 μm),流动相A:0.2%KH2PO4+0.08%四丁基硫酸氢铵混合溶液(pH 3.0)；流动相B:0.2%KH2PO4+0.08%四丁基硫酸氧铵混合溶液(以1 mol/NaOH调至pH 7.5)；流动相C:甲醇.梯度洗脱,同时改变流速和检测波长.血浆及RBC样品以HClO4沉淀蛋白予以净化.选用甲氧苄啶(TMP)为内标,以峰面积比内标法进行定量分析,以基线扣除法计算外源性PCr、Cr和相关ATP浓度.在上述色谱条件下,血浆及RBC中PCr、Cr、ATP及TMP达基线分离,不受基质干扰,PCr在10～7500 g/L(血浆)、10～2500 mg/L(RBC),Cr和ATP在10～1500 mg/L(血浆)、10～750 mg/L(RBC)范围内线性关系良好(r2>0.99)；3种分析物QC样品的日内和日间精密度(RSD)均小于10%；回收率均大于92%.采用本方法测定了静脉和口服给药PCr后大鼠血浆和RBC中3种分析物浓度,结果满意.
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