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目的 :通过现场和实验室试验检测,了解山银花不同月份不同部位绿原酸含量。方法:采用水提法、乙醇回流提取法提取,旋转蒸发仪浓缩,高效液相色谱法(LC-10AT)检测绿原酸含量。结果:山银花花蕾及带初开的花原药材绿原酸含量4月、6月、7月分别为3.0%、5.1%、2.8%,醇提法浓缩液分别为4.8%、12.3%、5.4%,山银花叶原药材绿原酸含量6月、7月、8月、9月、10月分别为0.5%、4.0%、3.7%、5.6%、5.7%,醇提法浓缩液分别为2.5%、10.0%、9.6%、9.9%、9.0%。结论 :山银花叶中绿原酸含量不低于中华人民共和国药典(2010版)山银花花蕾及带初开的花项下绿原酸含量(2%),山银花叶有开发应用价值,醇提法优于水提法.
OBJECTIVE: To understand the contents of chlorogenic acid in different parts of Lonicera japonica through field and laboratory tests. Methods: Water extraction, ethanol reflux extraction, concentration by rotary evaporator, and determination of chlorogenic acid by high performance liquid chromatography (LC-10AT). Results: The contents of chlorogenic acid in flower buds and flowers of Huayuansha with 3.0%, 5.1% and 2.8% respectively in April, June and July were 4.8% and 12.3% respectively, 5.4% respectively. The contents of chlorogenic acid in the original medicinal materials of Y. cerevisiae were 0.5%, 4.0%, 3.7%, 5.6% and 5.7% in June, July, August, September and October respectively. 2.5%, 10.0%, 9.6%, 9.9%, 9.0%. Conclusion: The content of chlorogenic acid in the leaves is not lower than that of the Chinese Pharmacopoeia (2010 edition), and the content of chlorogenic acid (2%) under the flowers with the beginning of the pharmacopoeia of the People’s Republic of China. Value, alcohol extraction is better than water extraction.