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目的探讨GSTO1基因多态性与饮水型地方性砷中毒易感性的关系。方法对新疆奎屯砷中毒病区慢性砷中毒患者96人,病区内对照组73人,以及非病区外对照组89人,应用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)技术对GSTO1基因rs4925及rs11509438多态位点进行检测,用聚合酶链反应-双对引物多态性(PCR-CTPP)方法对GSTO1基因rs11509437位点进行检测,分析不同基因型与砷中毒发病风险的关系。结果 GSTO1基因rs4925、rs11509438及rs11509437位点的基因型以及等位基因频率在三组间的分布差异均无统计学意义(P>0.05),GSTO1基因rs4925、rs11509438及rs11509437位点的基因多态性在不同砷暴露人群中的发病风险,差异均无统计学意义(P>0.05)。结论 GSTO1基因rs4925、rs11509438及rs11509437位点可能与新疆饮水型地方性砷中毒无关联。
Objective To investigate the relationship between GSTO1 gene polymorphism and susceptibility to endemic arsenism in drinking water. Methods 96 patients with chronic arsenic poisoning in Kuitun arsenism area of Xinjiang, 73 patients in control group and 89 patients in non-disease area were enrolled in this study. PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) ) Technique was used to detect rs4925 and rs11509438 polymorphisms of GSTO1 gene. The genotypes of rs11509437 of GSTO1 gene were detected by polymerase chain reaction-double-pair primer-pair polymorphism (PCR-CTPP) The relationship between risk. Results There were no significant differences in the distribution of genotype and allele frequencies of rs4925, rs11509438 and rs11509437 in GSTO1 among the three groups (P> 0.05). The polymorphisms of rs4925, rs11509438 and rs11509437 in GSTO1 There was no significant difference in the risk of developing arsenic among different groups (P> 0.05). Conclusion The loci of rs4925, rs11509438 and rs11509437 of GSTO1 gene may not be associated with drinking water endemic arsenism in Xinjiang.