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目的:了解海藻酸钙-聚赖氨酸-海藻酸钙(APA)微囊化牛肾上腺嗜铬细胞(APA-BCC)植入蛛网膜下腔后患者的免疫反应。方法:实验于2002-07/2003-02在解放军总医院老年医学研究所细胞生物学研究室完成。采用腰椎穿刺术,将APA-BCC注入20例中、重度癌痛患者L3～4或L4～5蛛网膜下腔内。检测患者移植前后的血象、免疫球蛋白、T细胞亚群及脑脊液。结果:移植后外周血中淋巴细胞比例较移植前无明显变化(P>0.05)。移植后7d复查16例患者的脑脊液,其中8例(50%)脑脊液中白细胞升高,范围在(15～700)×106L-1犤(198±248)×106L-1犦。但外周血免疫球蛋白、T细胞亚群较移植前无明显变化。结论:APA-BCCs微胶囊植入癌痛患者蛛网膜下腔,未引起宿主明显的免疫反应,表明APA微囊具有良好的免疫隔离作用。
OBJECTIVE: To investigate the immune response of patients with calcium alginate-polylysine-alginate (APA) microencapsulated bovine adrenal chromaffin cells (APA-BCC) implanted into the subarachnoid space. Methods: The experiment was performed in the Cell Biology Laboratory of the Institute of Geriatrics, PLA General Hospital from July 2002 to February 2003. APA-BCC was injected into the subarachnoid space of L3 ~ 4 or L4 ~ 5 in 20 patients with moderate to severe cancer pain using lumbar puncture. Blood samples, immunoglobulins, T-cell subsets and cerebrospinal fluid were measured before and after transplantation. Results: The proportion of peripheral blood lymphocytes after transplantation did not change significantly compared with those before transplantation (P> 0.05). Cerebrospinal fluid (CSF) of 16 patients was examined at 7 days after transplantation. Leukocytes in cerebrospinal fluid were increased in 8 cases (50%), ranging from (15 to 700) × 106L-1 犤 (198 ± 248) × 106L-1 犦. However, peripheral blood immunoglobulin, T cell subpopulations had no significant changes before transplantation. CONCLUSION: APA-BCCs microcapsules are implanted into the subarachnoid space of patients with cancer pain. The APA-BCCs microcapsules have no significant immune response, indicating that the APA-BCCs microcapsules have a good immune isolation effect.