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采用ＲＴＰＣＲ 技术对ＣＭＬ 患者骨髓细胞１４ ｄ 的细胞集落群和部分１４ ｄ 或２８ ｄ 的单个细胞集落ｂｃｒ／ａｂｌ ｍ ＲＮＡ 表达进行分析。结果显示：所检测的骨髓细胞集落ｂｃｒ／ａｂｌ 基因的表达均呈阳性，即为ＣＭＬ 集落，与细胞遗传学分析结果一致。该方法为研究ＣＭＬ 造血前体细胞基因表达及其诊断、治疗监测提供了灵敏、有效的分析手段；并可作为研究ＣＭＬ 发病、治疗及筛选抗ＣＭＬ 药物较为理想的方法。
RT-PCR was used to analyze the expression of bcr/abl mRNA in 14 days of bone marrow cells in CML patients and single cell colonies in 14 days or 28 days. The results showed that the expression of the bcr/abl gene in the detected bone marrow cells was positive, that is, CML colonies were consistent with the cytogenetic analysis results. The method provides a sensitive and effective analytical method for the study of gene expression and its diagnosis and therapeutic monitoring of hematopoietic progenitor cells in CML. It can be used as an ideal method for studying the pathogenesis, treatment and screening of anti-CML drugs in CML.