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目的:检测酸敏感离子通道(ASICs)在PC12细胞中的表达情况。方法:采用RT-PCR、Westernblot以及单细胞膜片钳记录方法检测ASICs在培养的PC12细胞中的表达情况。结果:PC12细胞中有ASICs蛋白的表达,且ASICs的6个亚基:ASIC1a,ASIC1b,ASIC2a,ASIC2b,ASIC3,ASIC4均有表达,给予酸诱导能产生电流。结论:PC12细胞上存在ASICs电流,且能被ASICs阻断剂阿米洛利所阻断,这为研究ASICs在神经退行性疾病中的作用提供了较为理想的细胞模型。
Objective: To detect the expression of acid sensitive ion channels (ASICs) in PC12 cells. Methods: The expression of ASICs in cultured PC12 cells was detected by RT-PCR, Western blot and single-cell patch-clamp recording. Results: The expression of ASICs in PC12 cells was observed. The six subunits of ASICs, ASIC1a, ASIC1b, ASIC2a, ASIC2b, ASIC3 and ASIC4, were expressed in PC12 cells and induced by acid. CONCLUSIONS: ASICs current exists on PC12 cells and can be blocked by ASICs blocker amiloride, which provides an ideal cell model for studying the role of ASICs in neurodegenerative diseases.