论文部分内容阅读
目的 :建立复方鼻用软膏中醋酸地塞米松含量测定方法。方法 :RP HPLC法 ,日本岛津Shim packVP ODS柱 (2 5 0mm× 4 .6mm ,5 μm) ,国产C18保护柱 ,以乙腈 水 (75∶2 5 )为流动相 ,检测波长为 2 5 4nm ,流速 0 .8ml·min-1。结果 :醋酸地塞米松回归方程为Y =2 6 35 0X +4731,r =0 .9995 ,平均回收率为 10 0 .0 9%。结论 :本法简单、准确 ,可用于复方鼻用软膏中醋酸地塞米松的快速定量分析
Objective: To establish a method for determination of dexamethasone acetate in compound nasal ointment. Methods: RP HPLC method was used. Shimadzu Shim pack VP ODS column (250 mm × 4.6 mm, 5 μm) made in China and C18 column made in China were prepared. The mobile phase consisted of acetonitrile and water (75:25), and the detection wavelength was 254 nm , Flow rate 0. 8ml · min-1. Results: The regression equation of dexamethasone acetate was Y = 2 6 35 0X + 4731, r = 0 .9995, the average recovery was 10 0. 09%. Conclusion: This method is simple and accurate and can be used for rapid quantitative analysis of dexamethasone acetate in compound nasal ointment