论文部分内容阅读
目的 观察山莨菪碱 (6 5 4 2 )和脂多糖 (LPS)共同处理单核细胞 (M )后对核因子 κB抑制蛋白(IκB)降解和核因子 κB(NF κB)活化的影响。方法 分离、培养M ,分为正常对照组 ;LPS刺激组 (LPS 10 μg/ml) ;6 5 4 2干预组 (6 5 4 2 10ng/ml预孵育 1h后加入LPS 10 μg/ml)。分别在刺激前 (0 )、刺激后 0 .2 5、0 .5、1和2h ,留取M。检测M中IκBα水平和核提取物中NF κB的活性。结果 LPS刺激组IκBα水平 15min开始降低 ,30min降到最低值 ;NF κB活性峰值显著高于刺激前和正常对照组 (P <0 .0 1)。 6 5 4 2干预后 ,IκBα水平最低值 ,显著高于LPS组 (P <0 .0 1)而NF κB活性虽较刺激前和正常对照组升高 ,但显著低于LPS组 (P <0 .0 1)。结论 LPS可诱导M的IκBα降解和NF κB激活 ,而 6 5 4 2能减少IκBα降解 ,抑制NF κB的活化。提示6 5 4 2在全身炎症反应综合症和急性肺损伤中可能具有治疗保护作用
Objective To observe the effects of monosamine (6,542) and lipopolysaccharide (LPS) on the degradation of nuclear factor kappa B inhibitor (IκB) and the activation of nuclear factor κB (NF κB) after treating mononuclear cells (M) Methods M was divided into normal control group, LPS stimulation group (LPS 10 μg / ml), and 6 5 4 2 intervention group (LPS 10 μg / ml after preincubation of 6 5 4 2 10 ng / ml for 1 h). Respectively before the stimulus (0), 0.52, 0.5, 0.5 and 2 h after stimulation, to retain M. The level of IκBα in M and the activity of NFκB in nuclear extracts were measured. Results The level of IκBα in LPS stimulation group began to decrease at 15 min and decreased to the lowest at 30 min. The peak of NF κB activity was significantly higher than that before stimulation and normal control group (P <0.01). After intervention, the lowest IκBα level was significantly higher than that in LPS group (P <0.01), while NF κB activity was significantly higher than that in LPS group (P <0. 05) .0 1). Conclusion LPS can induce the degradation of IκBα and activation of NF κB, while 6,542 can reduce the degradation of IκBα and inhibit the activation of NF κB. Tip 6 5 4 2 may have therapeutic protection in systemic inflammatory response syndrome and acute lung injury