论文部分内容阅读
从自主开发的近3000对烟草SSR引物中随机选出30对SSR引物,对38份晾晒烟种质资源的遗传关系进行了分析。在38份供试材料中共检测出173个等位基因,每对SSR引物可检测到的等位基因数为2～11个,平均为5.77个;遗传相似系数(GS)的变化范围为0.140～0.928,平均为0.534。表明38份晾晒烟的遗传多样性丰富,遗传差异较大,亲缘关系较远。聚类分析表明,在L1(GS=0.165)处可将38个品种分为2个类群,即晾晒烟类群和美国从烟草起源地收集的烟草(TI,tobaccoinstruction)类群;晾晒烟类群又可进一步分为4个亚类,其聚类结果与所期望的结果基本一致。表明SSR是一种有效、稳定和可靠的分子标记,能较好地从分子水平上揭示晾晒烟种质资源的遗传背景和亲缘关系。
Thirty SSR primers were randomly selected from nearly 3000 pairs of tobacco SSR primers developed independently to analyze the genetic relationship of 38 germinating tobacco germplasm resources. A total of 173 alleles were detected in 38 tested cultivars. The average number of alleles detected per SSR primer pair was 2 to 11 with an average of 5.77. The genetic similarity coefficient (GS) ranged from 0.140 ~ 0.928, averaging 0.534. The results showed that 38 copies of dried tobacco had rich genetic diversity, large genetic difference and distant genetic relationship. Cluster analysis showed that 38 cultivars could be divided into two groups at the level of L1 (GS = 0.165), that is, the dried tobacco group and the United States tobacco (TI) tobacco collection collected from the tobacco origin; Divided into four sub-categories, the clustering results and the expected results are basically the same. It indicated that SSR is an effective, stable and reliable molecular marker, which can better reveal the genetic background and genetic relationship of air-cured tobacco germplasm resources at the molecular level.