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【目的】初步探讨植物乳杆菌LpT1和LpT2的体外降胆固醇机制。【方法】以MRS、MRS+CH、MRS+CH+S和MRS+CH+N四种培养基为基础,接种植物乳杆菌LpT1和LpT2进行培养,通过分析比较培养基上清液、菌体沉淀和菌体细胞内部胆固醇含量以及接种和未接种两种情况上清液、沉淀和细胞内胆固醇总量变化,推测植物乳杆菌体外降胆固醇机制。【结果】乳酸菌体外降胆固醇存在非代谢和代谢降解两条途径,非代谢途径与共沉淀作用和菌体吸收有关。代谢降解是由于植物乳杆菌在生长过程中产生了特殊的酶系,从而将胆固醇降解成其他物质,导致其含量降低。【结论】研究结果为进一步研究植物乳杆菌体外降胆固醇的机制奠定了良好基础。
【Objective】 To investigate the cholesterol-lowering mechanism of LpT1 and LpT2 in vitro. 【Method】 Lactobacillus plantarum LpT1 and LpT2 were inoculated on the basis of four kinds of MRS, MRS + CH, MRS + CH + S and MRS + CH + N mediums, respectively. And the intracellular cholesterol content of cells and inoculated and uninoculated two cases of supernatant, sedimentation and intracellular total cholesterol changes speculated that Lactobacillus plantarum cholesterol lowering mechanism. 【Result】 Lactobacillus there were two mechanisms of non-metabolism and metabolic degradation of cholesterol in vitro. Non-metabolic pathway was related to coprecipitation and cell uptake. Metabolic degradation is due to Lactobacillus plantarum in the growth process produced a special enzyme system, which will cholesterol degradation into other substances, resulting in reduced levels. 【Conclusion】 The results provide a good foundation for further study on the mechanism of Lactobacillus plantarum cholesterol lowering.