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应用体外培养的红内期食蟹猴疟原虫(Pc)与感染猴体的Pc制备的抗原,用ELISA测定间日疟抗体的效果进行了比较。两种抗原对发病19～90d的64例间日疟病人的阳性率均为96.9％,抗体滴度的分布也基本一致。用体外培养的Pc制备可溶性抗原具有容易获得成熟疟原虫的优点,且可根据需要随时培养和制备新鲜抗原,解决了Pc抗原长期贮存活性易受影响的问题,并证实了PVC薄膜作载体和HRP无毒新底物TMBS用于ELJSA检测间日疟抗体是可行的。
Plasmodium vivax (Pc) in vitro and Pc-derived antigens from infected monkeys were used to evaluate the efficacy of anti-Japanese malaria antibodies by ELISA. The positive rates of two antigens in 96 cases of P. falciparum between 19 and 90 days were 96.9%, and the distribution of antibody titer was basically the same. Preparation of soluble antigens from pc in vitro culture has the advantages of easy access to mature malaria parasite and the ability to grow and prepare fresh antigens at any time as needed to solve the problem of long-term storage activity of Pc antigens and to prove that PVC film is a carrier and HRP TMBS, a nontoxic new substrate, is feasible for ELJSA detection of Plasmodium vivax.