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目的:观察通心络对自发性糖尿病(GK)大鼠心肌Ⅰ型和Ⅲ型胶原阳性表达及含量、细胞转化生长因子(TGF-β1)含量的影响。方法:4月龄GK大鼠30只,随机分为GK对照组、通心络大、小剂量组,另取4月龄Wistar大鼠10只作正常对照,分别灌胃12周。灌胃结束后,制备心肌切片,免疫组化染色,计算胶原I、胶原Ⅲ阳性面密度比值以代表表达程度;取心肌匀浆,酶联免疫方法测定心肌Ⅰ、Ⅲ型胶原及TGF-β1含量。结果:与同月龄正常大鼠相比较,GK大鼠血糖明显增高(P<0.01),通心络药物组有降低趋势,但无差异(P>0.05)。与正常大鼠比较,GK大鼠心肌胶原Ⅰ阳性面密度比值明显增高(P<0.01),胶原Ⅲ阳性面密度比值明显降低(P<0.01),心肌匀浆胶原Ⅰ含量明显增高(P<0.01),胶原Ⅲ含量降低(P<0.05),心肌胶原Ⅰ/Ⅲ比值明显增高(P<0.01),TGF-β1含量降低(P<0.01);与7月龄GK大鼠比较,通心络组胶原Ⅰ阳性面密度明显降低(P<0.01),高、低剂量组之间有差异(P<0.05),胶原Ⅲ阳性面密度明显升高(P<0.01);胶原Ⅰ含量明显降低(P<0.01),胶原Ⅲ含量明显提高(P<0.01),胶原Ⅰ/Ⅲ比值降低(P<0.01),TGF-β1含量明显提高(P<0.01),高、低剂量组之间无差异(P>0.05)。结论:通心络可降低GK大鼠心肌胶原Ⅰ阳性表达,Ⅰ型胶原含量,升高胶原Ⅲ阳性表达,Ⅲ型胶原含量,降低胶原Ⅰ/Ⅲ比值,升高TGF-β1含量,具有改善心肌间质网络重构作用。
OBJECTIVE: To observe the effect of Tongxinluo on the positive expression and content of type I collagen and type III collagen and the content of transforming growth factor (TGF-β1) in myocardium of spontaneously diabetic rats. Methods: Thirty 4-month-old GK rats were randomly divided into GK control group, Tongxinluo large-dose group and small-dose group. Ten-month-old Wistar rats were also taken as normal controls and were given intragastric administration for 12 weeks. At the end of gavage, myocardial slices were prepared and immunohistochemically stained. The ratio of positive surface density of collagen I and collagen III was calculated to represent the degree of expression. Myocardium homogenate and enzyme-linked immunosorbent assay were used to determine the contents of type I and III collagen and TGF-β1 in myocardium. . RESULTS: Compared with normal rats of the same age, blood glucose in GK rats was significantly higher (P<0.01). Tongxinluo drug group had a decreasing trend, but there was no difference (P>0.05). Compared with normal rats, the positive ratio of myocardial collagen I surface density in GK rats was significantly higher (P<0.01), the ratio of positive area ratio of collagen III was significantly lower (P<0.01), and the content of collagen I in myocardial homogenates was significantly higher (P<0.01). ), collagen III content decreased (P <0.05), myocardial collagen I / III ratio was significantly higher (P <0.01), TGF-β1 content decreased (P <0.01); compared with 7-month-old GK rats, Tongxinluo group The positive area density of collagen I was significantly decreased (P<0.01), there was a difference between high and low dose groups (P<0.05), and the positive area density of collagen III was significantly higher (P<0.01); collagen I content was significantly lower (P< 0.01), Collagen III content increased significantly (P <0.01), collagen I / III ratio decreased (P <0.01), TGF-β1 content increased significantly (P <0.01), there was no difference between high and low dose groups (P> 0.05). Conclusion: Tongxinluo can reduce the positive expression of collagen I, the content of type I collagen, increase the positive expression of collagen III, increase the content of type III collagen, decrease the ratio of collagen I/III, and increase the content of TGF-β1 in myocardium of GK rats. Interstitial network reconstruction.