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介绍了漆酚树脂吸附金属离子后对漆树酶的固定化方法，比较了Fe~（3＋）和Al~（3＋）、漆酚树脂和漆酚-水杨酸接枝树脂对漆树酶的固定化活性，结果显示，漆酚-水杨酸－Al螯合树脂对漆树酶的固定化活力最高。测定了溶液pH、环境温度对固定化漆树酶活性的影响及固定化漆树酶的重复使用性。另外，测定了固定化漆树酶的米氏常数，K_m~′＝4．9×10~（-3），并讨论了K_m~′小于天然漆树酶的米氏常数的原因。
In this paper, the immobilization of lacterase on the adsorption of metal ions by urushiol resin was introduced. The immobilization of lactease by Fe ~ (3+) and Al ~ (3+), urushiol resin and urushiol-salicylic acid grafted resin was compared Activity, the results show that urushi phenol - salicylic acid - Al chelate resin immobilized the highest activity of the enzyme. The effects of solution pH and ambient temperature on the immobilized enzyme activity and the reusability of the immobilized enzyme enzyme were measured. In addition, the Michaelis constant of immobilized lactease was determined, Km ~ ’= 4.9 × 10 ~ (-3), and the reason why Km ~’ was less than that of natural laccase was discussed.