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探讨了连续给予和汉药对肝脏与小肠中CYP活性的影响以及对并用药物体内动态的影响。方法：①和汉药的预处理：对Wistar系雄性大鼠(7周龄)每天2次经口给予男宝、柴苓汤、补中益气汤的混悬液,连续给予1周。给药结束后禁食一夜,用于实验。大鼠小肠、肝微粒体按常规方法制备。②体外代谢实验：在NADPH生成系存在下,将微粒体(500μg)与各种浓度的硝苯地平37℃培
The effects of continuous administration and Chinese medicine on the CYP activity in the liver and small intestine and the effects on the in vivo dynamics of the combined drugs were investigated. Methods: 1 Pretreatment with Chinese medicine: Wistar male rats (7 weeks old) were orally given a suspension of male treasure, chai yin decoction, and Buzhong Yiqi Decoction twice a day for 1 week. Fasting overnight after the administration was used for the experiment. Rat small intestine, liver microsomes were prepared by conventional methods. 2In vitro metabolism experiments: Microsomes (500μg) were incubated with various concentrations of nifedipine at 37°C in the presence of NADPH production lines.