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目的:观察脱氧雪腐镰刀菌烯醇(DON)、N-亚硝基化合物(NOC)对昆明小鼠肝脏组织损伤及潜在致癌作用。方法:128只小鼠随机分成4组,每组32只,利用隔日灌胃法,给小鼠染毒DON、NOC。DON组染毒剂量为每次0.5 mg/kg,NOC组染毒剂量为每次0.25 mg/kg,DON+NOC组染毒剂量为每次DON 0.5 mg/kg+NOC 0.25 mg/kg,对照组给予等体积生理盐水。60 d后处死小鼠,取肝脏组织制作HE病理切片,观察肝脏组织病理改变。结果:实验组动物肝脏大片坏死、大片脂肪变性、小灶区异型、双核肝细胞阳性率与对照组比较有统计学意义(P值依次分别为0.001 8、0.000 7、0.009 3和0.022 3)。DON+NOC组出现的大片脂肪变性分别与DON组、NOC组进行四格表χ2检验,差异有统计学意义,P=0.005 8。结论:DON、NOC对肝脏有明显损伤,两者联合可加重肝脏损伤,且具有潜在的致癌效应。
OBJECTIVE: To observe the damage and potential carcinogenic effects of deoxynivalenol (DON) and N-nitroso compound (NOC) on liver tissues of Kunming mice. Methods: 128 mice were randomly divided into 4 groups, 32 in each group. The mice were exposed to DON and NOC by gavage every other day. The dose of DON was 0.5 mg/kg, the dose of NOC was 0.25 mg/kg, and the dose of DON+NOC was DON 0.5 mg/kg+NOC 0.25 mg/kg. Give an equal volume of normal saline. After 60 days, the mice were sacrificed and liver tissues were taken for HE pathological examination to observe the histopathological changes of the liver. RESULTS: There were statistically significant differences in the necrosis, large steatosis, focal lesions, and binuclear hepatocytes positive rates between the experimental group and the control group (P values were 0.001 8, 0.000 7, 0.009 3, and 0.022 3, respectively). The large fatty degeneration in the DON+NOC group was compared with the DON and NOC groups. The difference was statistically significant (P=0.005 8). Conclusion: DON and NOC have obvious damage to the liver, and the combination of the two can aggravate liver injury and have potential carcinogenic effects.