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Objective: To study the mechanism of cancer, the DNA for BAC was cloned from an ascites hepatoma cell line Hca-F25/CL-16A3 using PCR. Methods: The nucleotide sequences were determined using ABI PRISMTM 377 DNA sequencer. The expression of bcl-2 and CDK4gene were determined using immunohistochemistry.Results: The sequences of BAC segment on HcaF25/CL-16A3 have nearly identical sequences with human BAC. The bcl-2 and CDK4 are highly expression on this cell line. Conclusion: The highly expression of bcl-2 and CDK4 may the one of mechanisms for tumor growth.