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目的:观察芪术颗粒对阿霉素肾病大鼠肝功能的影响。方法:SD大鼠随机分为6组:空白组,模型组,泼尼松(10 mg·kg-1)组和芪术颗粒(按生药量计,5,10,20 g·kg-1)剂量组;除空白组外,其他各组采用阿霉素静脉注射的方法制备大鼠阿霉素肾病模型;连续给药4周,进行血清蛋白、血脂、肝、肾功能的检测以及HE染色病理观察和免疫组化分析肾脏纤维连接蛋白(FN)表达。结果:与空白组比较,模型组动物总胆固醇(TCH)和甘油三酯(TG)水平均明显升高(P<0.01),血清白蛋白以及肝、肾功能下降,肝、肾组织病理形态明显异常,肾脏FN表达明显升高(P<0.01);与模型组比较,芪术颗粒可明显降低模型动物血清TCH和TG水平(P<0.05,P<0.01),提高血清白蛋白水平,改善肝、肾功能,减轻肝、肾的组织病理性损伤,同时降低肾脏FN表达。结论:芪术颗粒长期给药对阿霉素肾病大鼠肝功能具有明显的保护作用。
Objective: To observe the effect of Qizhu Granule on liver function of adriamycin-induced nephropathy rats. Methods: SD rats were randomly divided into 6 groups: blank group, model group, prednisone (10 mg · kg -1) group and Qizhu Granule (5,10 and 20 g · kg -1) Doxorubicin was injected into rats in other groups except for the blank group. The adriamycin-induced nephropathy model was established by intravenous injection for four weeks. Serum protein, lipids, liver and renal function were detected by HE staining Observation and immunohistochemistry analysis of renal Fibronectin (FN) expression. Results: Compared with the blank group, the levels of total cholesterol (TCH) and triglyceride (TG) in the model group were significantly increased (P <0.01), serum albumin, liver and kidney function decreased, the pathological changes of liver and kidney were obvious (P <0.01). Compared with the model group, Qizhu Granule could significantly reduce the level of serum TCH and TG (P <0.05, P <0.01), raise serum albumin level and improve liver , Renal function, reduce liver and kidney histopathological damage, while reducing renal FN expression. Conclusion: Long-term administration of Qizhu granule has obvious protective effect on hepatic function of adriamycin-induced nephropathy rats.