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以‘紫梗籽银’桂(Osmanthus fragrans’Zigengziyin’)种子为试材,对其内果皮、种实内源抑制物质进行提取、生物测定,并采用GC-MS进行鉴定。结果表明:1‘紫梗籽银’桂种子内果皮、种实均含有抑制种子萌发和幼苗生长的物质,且内果皮、种实中各分离相的抑制效果均不相同。其中石油醚相抑制作用最弱,乙酸乙酯相和甲醇相抑制作用较强。2GC-MS鉴定结果显示,‘紫梗籽银’桂种子内果皮、种实中存在多种抑制物质,包括棕榈酸、油酸、9,12-十八碳二烯酸等发芽抑制物质,主要为脂肪酸类物质。
Osmanthus fragrans’Zigengziyin ’seeds were used as experimental material to extract and bioassay the endogenous inhibitory substances of endocarp and seed, and identified by GC-MS. The results showed that the inner pericarp of 1 ’purple sedge seed’ Gui seeds contained inhibiting substances of seed germination and seedling growth, and the inhibitory effects of the separated phases in endocarp and seed were different. Petroleum ether phase of which the weakest inhibition, ethyl acetate and methanol phase stronger inhibition. The results of identification by 2GC-MS showed that there were many inhibitory substances in the seed coat of ’Zizyphus sibiricus’ seeds, including sprouting inhibiting substances such as palmitic acid, oleic acid and 9,12-octadecadienoic acid, For fatty acids.