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【目的】克隆绿僵菌异戊烯基转移酶(Mpt)基因,了解该基因的结构和表达特征。【方法】采用SMART(Switching Mechanism At 5′end of RNA Transcript)技术和PCR技术,扩增Mpt基因全长cDNA序列和DNA序列,用qRT-PCR方法分析该基因在绿僵菌不同侵染时期的表达谱。【结果】Mpt基因含2个外显子和1个内含子,CDS区为1026bp(GenBank登录号GU271134),编码341个氨基酸;qRT-PCR分析表明,该基因在绿僵菌侵染的不同时期表达水平有显著差异,特别是在寄主昆虫体内生长的后期高表达。【结论】首次克隆了绿僵菌的Mpt基因,弄清了该基因具有在侵染后期高表达等特征,为研究该基因的功能奠定了基础。
【Objective】 The objective of this study was to clone the Metopentyltransferase (Mpt) gene of Metarhizium anisopliae and understand its structure and expression characteristics. 【Method】 The full-length cDNA sequence and DNA sequence of Mpt gene were amplified by using SMART (Switching Mechanism At 5’end of RNA Transcript) technology and PCR. The qRT-PCR method was used to analyze the expression of Mpt gene in different stages of Metarhizium anisopliae Expression profile. 【Result】 The Mpt gene contained two exons and one intron. The CDS region was 1026 bp (GenBank accession number GU271134) and encoded 341 amino acids. The qRT-PCR analysis showed that the Mpt gene was expressed in M. anisopliae There was a significant difference in the expression level during the period, especially in late growth of host insects. 【Conclusion】 The Mpt gene of Metarhizium anisopliae was cloned for the first time, and it was clarified that it has the characteristics of high expression in the late stage of infection, which laid the foundation for the study of the function of this gene.