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观察丹参对乳鼠心肌细胞凋亡的影响,并探索其机理。采用体外培养的wistar乳鼠心肌细胞缺糖缺氧6h,用心肌酶检测其代射及流式细胞术检测心肌细胞凋亡。结果显示缺糖缺氧组心肌细胞凋亡数及酶指数显著高于正常对照组,大、小剂量丹参液组心肌细胞凋亡及酶数量明显下降,与缺糖缺氧组比较有显著性差异。说明丹参对缺糖缺氧心肌细胞凋亡有明显的抑制作用。
Observe the effect of salvia miltiorrhiza on cardiomyocyte apoptosis in neonatal rats and explore its mechanism. The in vitro cultured wistar neonatal cardiomyocytes were subjected to hypoxia and hypoxia for 6 h. Myocardial enzymes were used to detect the cardiomyocytes and flow cytometry was used to detect cardiomyocyte apoptosis. The results showed that the number of myocardial cell apoptotic cells and enzyme index in the hypoxia-hypoxia group was significantly higher than that in the normal control group. The number of myocardial cell apoptosis and the number of enzymes decreased significantly in the large and small dose Danshen liquid group, and there was a significant difference compared with the hypoxia-hypoxia group. . It shows that salvia miltiorrhiza has a significant inhibitory effect on the apoptosis of cardiomyocytes with hypoxia and hypoxia.