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目的通过动物模型的构建,为大肠癌研究提供可靠的实验依据。方法以二甲基肼诱发Wistar大鼠大肠癌,分别于给药后12、18、24周处死动物,观察各时期实验组及对照组大鼠大肠粘膜的病理变化。结果诱癌过程中大鼠大肠依次出现轻、中、重度不典型增生。诱癌12周时实验组未见肿瘤形成;18周肿瘤的发生率为60%,50%为大肠癌;24周肿瘤的发生率为100%,大肠癌的发生率为80%。结论二甲基肼诱导大鼠大肠癌成功率高,与人类大肠癌发生、发展相似,可用于模拟人类大肠癌的实验研究。
Objective To establish a reliable animal model for the study of colorectal cancer. Methods Dimethylhydrazine was used to induce colorectal cancer in Wistar rats. Animals were sacrificed at 12, 18 and 24 weeks after administration respectively. The pathological changes of the large intestine mucosa in experimental group and control group were observed. Results The large intestine of rats in the course of induction of cancer showed mild, moderate and severe atypical hyperplasia. At 12 weeks after induction of cancer, no tumor was found in the experimental group. The incidence of tumors at 18 weeks was 60% and 50% was colorectal cancer. The incidence of tumors at 24 weeks was 100% and the incidence of colorectal cancer was 80%. Conclusion Dimethylhydrazine induced a high success rate of colorectal cancer in rats, similar to that of human colorectal carcinomas. It may be used to simulate the experimental study of human colorectal cancer.