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近年来利用低温贮藏培养的细胞或分生组织以保存或运输有意义的种质已受到重视。王大元等通过三年继代培养,对由胚愈伤组织得到的柳橙胚状体和胚芽进行了试管保存,取得了初步成功。近来还出现了“组织培养贮藏技术”( TCST)的专门术语。Cameson提出,美国准备研究用组织培养技术来保存柑桔种质。为了加快茎椰菜( Brassica oleracea var.italica)的育种进程,我们已在留种、抢救种质等方面应用了组织培养技术,同时,对利用试管苗在室温下长期保存种质作了初步观察。在此基础上,进行了室温下不同自然光照保存的试验并观察了试管苗的生长动态。期望通过此研究,能应用组织培养技术为茎椰菜优良种质常规保存提供一种简便有效的补充手段,更好地为育种服务。
In recent years, the use of cryogenic storage of cultured cells or meristems to preserve or transport meaningful germplasms has received significant attention. Wang Da-Yuan and other three generations of subculture, embryoid callus obtained from embryonic callus and embryos were preserved in vitro, and achieved initial success. Recently there has also been a term “Tissue Culture Storage Technology” (TCST). Cameson suggested that the United States is ready to study the use of tissue culture techniques to preserve citrus germplasm. In order to speed up the process of breeding Brassica oleracea var.italica, we have applied tissue culture techniques to plant seeds, rescued germplasm, and conducted a preliminary observation on long-term preservation of germplasm at room temperature using in vitro plantlets. On this basis, different natural light preservation tests were conducted at room temperature and the growth dynamics of test tube seedlings was observed. It is hoped that through this research, tissue culture technology can be used to provide a convenient and effective supplementary means for the routine preservation of superior germplasm of broccoli and to better serve breeding.