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目的:了解目前常规清洗消毒胃镜方法—三步法对幽门螺杆菌(HP)清除效果。方法:采用聚合酶链反应(PCR)及HP培养法对42例患者胃镜检查后,清洗消毒前后的胃镜活检通道冲洗液进行了检测。结果:31例(73.8%)为HP感染者,PCR检测清洗消毒前冲洗液有26份(83.9%)为阳性,清洗消毒后仍有9份(34.6%)为阳性;而培养在清洗消毒前有12份(38.7%)为阳性,清洗消毒后均为阴性。另有2例患者胃窦粘膜组织Warthin—Slarry(W.S)染色HP为阴性者,胃镜检查后,其清洗消毒前冲洗液PCR检测为阳性,清洗消毒后均为阴性。结论:目前胃镜清洗消毒方法对HP的灭活是有效的,但仍有HP的菌体或DNA片段残留,应该加强对胃镜的冲洗。
Objective: To understand the current routine cleaning and disinfecting endoscopy - three-step method of Helicobacter pylori (HP) scavenging effect. Methods: Polymerase chain reaction (PCR) and HP culture were used to detect gastroscopic biopsy fluid in 42 patients before and after gastroscopy. Results: Totally 31 (73.8%) patients were infected with HP, 26 (83.9%) were positive after PCR test, and 9 (34.6%) were still positive after cleaning and disinfection. 12 (38.7%) were positive, negative after cleaning and disinfection. Another two cases of gastric mucosa Warthin-Slarry (W.S) staining HP was negative, gastroscopy, the washing and disinfection before washing liquid PCR test was positive, after washing and disinfection were negative. Conclusion: At present, gastroscopic cleaning and disinfection method is effective for HP inactivation, but still there are still residual cells or DNA fragments of HP, and the gastroscope washing should be strengthened.