一、菌种配制适宜香菇一级种的培养基配方是:马铃薯250克、葡萄糖25克、琼脂20克、清水1000毫升,或者是黄豆粉40克、葡萄糖20克、琼脂20克、清水1000毫升。用木屑作主料的二级种培养基配方是:木屑78%、麦麸20%、蔗糖1%、石膏粉1%,清水适量,使料水比为1:1.2。原种再转到相同的培养基上进行扩大栽培即为栽培种。每瓶原种可接栽培种60～80瓶,菌丝生长适宜温度范围为5～32℃,以22～25℃最合适,空气相对湿度以70%为宜。 First, the strain preparation suitable for a mushroom medium formula is: 250 grams of potato, 25 grams of glucose, 20 grams of agar, 1000 ml of water, or 40 grams of soybean meal, glucose 20 grams, 20 grams of agar, 1000 ml of water . The second medium with sawdust is formulated as follows: 78% of sawdust, 20% of wheat bran, 1% of sucrose, 1% of gypsum powder, proper amount of water and 1: 1.2 of material to water ratio. The original species then transferred to the same medium to expand cultivation is cultivated. Each bottle of original species can be cultivated 60 to 80 bottles of mycelium growth suitable temperature range of 5 ~ 32 ℃ to 22 ~ 25 ℃ the most appropriate, the air relative humidity of 70% is appropriate.