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子孢子外周(CS)蛋白是一些疟原虫种的保护性抗原。抗CS蛋白的单克隆抗体能与另外二条细胞内多肽(IS_1和IS_2)发生免疫活性沉淀,这二条多肽得自[~(35)S]甲硫氨酸代谢标记的原虫抽提液中。本文作者通过双相电泳和反相高压液相层析,比较了来自伯氏疟原虫,诺氏猴疟原虫,食蟹猴疟原虫和人恶性疟原虫的CS和IS。在还原和非还原的情况下,这些疟原虫种的CS,IS_1和IS_2的分子量在42,000～67,000之间。它们的等电点值范围为4.7到6.0。由每一种疟原虫所分离出来的CS和IS蛋白能与各自的单克隆抗体反应,产生非常类似的肽段图谱。这些结果证明IS是CS的细胞内前体,而且因CS
Sporozoite peripheral (CS) proteins are some of the protective antigens of Plasmodium species. Monoclonal antibodies against CS protein were immunoprecipitated with two other intracellular polypeptides (IS_1 and IS_2), which were obtained from the parasite extract metabolized by [~ (35) S] methionine. The authors compared CS and IS from Plasmodium berghei, Plasmodium naivensis, Plasmodium cerci, and Plasmodium falciparum by biphasic electrophoresis and reverse phase high pressure liquid chromatography. In the case of reduction and non-reduction, the molecular weights of CS, IS_1 and IS_2 of these Plasmodium species ranged from 42,000 to 67,000. Their isoelectric point range is 4.7 to 6.0. The CS and IS proteins isolated from each of the plasmodium species react with the respective monoclonal antibody to produce a very similar peptide map. These results demonstrate that IS is an intracellular precursor of CS and is due to CS