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目的:研究内质网应激相关蛋白CHOP/GADD153在子痫前期患者胎盘组织中的表达及其意义。方法:选择2009年6月～2010年6月在南京医科大学第一附属医院产科住院分娩的子痫前期患者30例(子痫前期组),以同期正常孕妇35例为对照组。采用RT-PCR技术、蛋白印迹(western-blot)法和免疫组化检测两组孕妇胎盘组织中CHOP/GADD153的mRNA与蛋白表达水平差异。结果:子痫前期组患者胎盘组织中CHOP/GADD153的mRNA与蛋白表达水平均显著高于相应孕周正常妊娠组。结论:CHOP/GADD153表达的增高与子痫前期的发病相关。
Objective: To study the expression and significance of endoplasmic reticulum stress-related protein CHOP / GADD153 in placenta of preeclampsia. Methods: Thirty patients with preeclampsia (preeclampsia group) delivered in the obstetrics and gynecology department of the First Affiliated Hospital of Nanjing Medical University from June 2009 to June 2010 were selected as the control group with 35 normal pregnant women in the same period. The differences of mRNA and protein levels of CHOP / GADD153 in placenta between two groups were detected by RT-PCR, western-blot and immunohistochemistry. Results: The levels of mRNA and protein of CHOP / GADD153 in placenta of preeclampsia group were significantly higher than those of normal pregnancy group. Conclusion: The increased expression of CHOP / GADD153 is associated with the pathogenesis of preeclampsia.