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目的 分析颌面部软组织爆炸伤早期愈合过程中血管内皮生长因子 (VEGF)及其受体Flt- 1的表达和变化 ,探讨VEGF在上述损伤愈合过程中的作用。方法 采用改进的家兔颌面部爆炸伤动物模型 ,以KTY - 0 4型雷管为爆炸源 ,复制颌面部爆炸损伤。选用ABC免疫组化染色法分别检测伤后 6h、1d、2d、3d、5d和 7d伤口组织中VEGF及其受体Flt- 1的表达 ,并与伤前组比较。结果 颌面部爆炸伤伤口组织中VEGF表达在伤后第一周内稳步上升 ,于伤后第 1d起和伤前组比较 ,差异明显 ,伤后第 3d开始 ,差异非常显著 (P <0 .0 1) ,伤后 7d达峰值。VEGF受体Flt- 1的表达则在伤后 3d内与伤前组比较无显著差异 ,伤后 5～ 7d其蛋白质的表达明显加强。结论 VEGF在颌面部爆炸伤后的表达与创伤愈合过程中新生血管形成的时间相似 ,其受体Flt- 1的表达也多发生于此时期 ,说明VEGF参与调节了创伤愈合过程中的血管再生阶段 ,对毛细血管的发生有积极促进作用
Objective To investigate the expression and changes of vascular endothelial growth factor (VEGF) and its receptor Flt-1 in early healing of maxillofacial soft tissue explosive injury and to explore the role of VEGF in the healing process. Methods An improved animal model of maxillofacial explosive injury in rabbits was established. KTY - 0 4 type detonator was used as an explosive source to replicate the maxillofacial explosive damage. The expression of VEGF and its receptor Flt-1 in wound tissues at 6h, 1d, 2d, 3d, 5d and 7d after wounding were detected by ABC immunohistochemical staining and compared with pre-injury group. Results The expression of VEGF in oral and maxillofacial explosive wounds increased steadily in the first week after injury, and there was a significant difference between the first day and the beginning of the injury. The difference was significant at the third day after injury (P <0. 0 1), reaching the peak on the 7th day after injury. The expression of Flt-1, a VEGF receptor, was not significantly different from the pre-injury group within 3 days after injury, and its protein expression was significantly enhanced 5-7 days after injury. Conclusion The expression of VEGF in maxillofacial explosive injury is similar to the time of neovascularization during wound healing. The expression of VEGF receptor Flt-1 also occurs frequently during this period, indicating that VEGF is involved in the regulation of vascular revascularization during wound healing Phase, the occurrence of capillaries have a positive role in promoting