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目的 掌握猕猴耳廓软骨细胞的体外分离、培养和鉴定技术 ,探讨耳廓软骨作为软骨细胞供区的可行性。材料与方法 :对 6只猕猴进行耳廓软骨取材、软骨细胞的分离 ,并行单层贴壁培养。通过倒置显微镜观察细胞生长情况并行细胞生长曲线的绘制 ;通过免疫组织化学染色对细胞分泌的基质成分进行鉴定。结果 :6只猕猴的耳廓软骨经分离后 ,获得了高纯度、高活性的软骨细胞 ,并成功地进行了体外培养 ;软骨细胞倍增时间为 98小时 ;免疫组织化学染色发现体外培养的软骨细胞具有分泌胶原基质的能力 ,但第三代细胞分泌Ⅰ、Ⅱ型胶原的能力无明显区别。结论 :利用猕猴耳廓软骨细胞体外分离及培养 ,能成功地获得具有体内活性的软骨细胞 ,耳廓软骨是一种易获取的软骨细胞供区
OBJECTIVE To master the isolation, culture and identification of rhesus aureus cartilage cells in vitro and to explore the feasibility of auricle cartilage as the donor area for chondrocytes. MATERIALS AND METHODS: A total of 6 macaques were auricular cartilage and chondrocytes were isolated and cultured in parallel with single layer. The growth of cells was observed by inverted microscope and the cell growth curve was drawn. The matrix components secreted by the cells were identified by immunohistochemical staining. RESULTS: The auricle cartilage of 6 macaques was isolated, and the chondrocytes with high purity and activity were obtained. The chondrocytes were successfully cultured in vitro. The chondrocyte doubling time was 98 hours. The chondrocytes cultured in vitro were identified by immunohistochemistry With the ability to secret collagen matrix, but the third-generation cells secrete type I, II collagen no significant difference in ability. Conclusion: The chondrocytes with in vivo activity can be obtained successfully by the isolation and culture of rhesus auricle cartilage cells in vitro. The auricle cartilage is an easily available chondrocyte for donor area