【摘 要】
：
p16INK4α基因的失活与多种肿瘤的发生和发展有联系.通过筛选小鼠基因组文库,获得长度为14.5kb的p16INKα基因组DNA片段.对上述14.5kb DNA测序后进行生物信息学分析表明:该片
【机 构】
：
第二军医大学医学遗传教研室,上海交通大学动物科学系
论文部分内容阅读
p16INK4α基因的失活与多种肿瘤的发生和发展有联系.通过筛选小鼠基因组文库,获得长度为14.5kb的p16INKα基因组DNA片段.对上述14.5kb DNA测序后进行生物信息学分析表明:该片段包含3个外显子,编码1个由168个氨基酸残基组成的多肽,其相对分子质量的理论计算值为17 941,有7个可能的磷酸化位点,说明p16INK4α蛋白的功能可能受到磷酸化的调控.该DNA片段的非编码区分布着大量短散布元件、长散布元件和简单重复序列,这样的结构为转座和同源重组提供了结构基础,提示了部分肿瘤细胞中p16INK4α基因缺失的可能原因.对第一外显子序列与已发表的相应序列比较发现其DNA序列和所编码的多肽存在多态性.
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