论文部分内容阅读
目的观察三氧化二砷(As2O3)对黑色素瘤B16细胞(B16细胞)的作用,探讨As2O3抗肿瘤的机制。方法Hoechst33258染色观察细胞凋亡特征;应用激光扫描共聚焦显微术(LSCM),结合特异性荧光探针Fluo-3/AM、H2DCF-DA和DAF-FM-DA,观察As2O3引起瘤细胞内钙离子(Ca2+)、活性氧(ROS)和一氧化氮(NO)的变化。结果50μmol/LAs2O3作用细胞12h后,Hoechst33258核染色可见典型凋亡细胞;细胞内Ca2+、ROS和NO含量明显高于对照组(P<0·01)。结论As2O3可诱导B16细胞发生凋亡,As2O3诱导凋亡可能与细胞内Ca2+、ROS和NO的增加有关。
Objective To observe the effect of arsenic trioxide (As2O3) on melanoma B16 cells (B16 cells) and explore the anti-tumor mechanism of As2O3. Methods Hoechst 33258 staining was used to observe the characteristics of cell apoptosis. Laser scanning confocal microscopy (LSCM) was used in combination with specific fluorescent probes Fluo-3/AM, H2DCF-DA and DAF-FM-DA to observe the intracellular calcium induced by As2O3. Changes in ions (Ca2+), reactive oxygen species (ROS), and nitric oxide (NO). Results After treated with 50μmol/LAs2O3 for 12h, typical apoptotic cells were seen by Hoechst33258 nuclear staining. The levels of intracellular Ca2+, ROS and NO were significantly higher than those in the control group (P<0.01). Conclusion As2O3 can induce apoptosis of B16 cells, and As2O3 induced apoptosis may be related to the increase of intracellular Ca2+, ROS and NO.