论文部分内容阅读
目的:通过变应原诱发大鼠变应性鼻炎(AR),观察鼻黏膜纤毛超微结构和鼻腔症状变化的特点。方法:采用卵清蛋白(OVA)为变应原激发大鼠鼻黏膜建立AR大鼠模型,建模成功后,每周2次鼻腔激发,分别至16 d、42 d、56 d、112 d、140 d。正常对照组在整个试验过程中以生理盐水代替OVA。观察变应原激发后的鼻黏膜纤毛超微结构改变以及检测鼻黏膜中浸润的嗜酸粒细胞(EOS)情况,同时观察变应原诱导的大鼠鼻腔流涕症状。结果:暴露变应原会引起大鼠鼻黏膜细胞纤毛排列紊乱,有的纤毛粘集成团向不同方向倾倒,上皮纤毛和绒毛多数脱落等超微结构进行性损害改变,EOS浸润先增多后降低;抓鼻次数变化无统计学意义,而鼻腔流涕症状起初一直加重,随着暴露变应原时间的延长出现鼻涕排除障碍。结论:AR大鼠模型在延长变应原诱导下鼻黏膜纤毛呈进行性损害;此外,长期变应原攻击下鼻腔症状变化可能与鼻黏膜纤毛结构进行性损害有关。
OBJECTIVE: To investigate the changes of nasal mucosa ultrastructure and nasal symptoms by allergen-induced allergic rhinitis (AR) in rats. Methods: The rat model of AR was established by using ovalbumin (OVA) as an allergen to stimulate the nasal mucosa of rats. After successful modeling, nasal challenge was induced twice a week to 16 d, 42 d, 56 d, 112 d, 140 d. The normal control group replaced normal saline with OVA throughout the experiment. To observe the ultrastructural changes of nasal mucosa after allergic provocation and to detect infiltration of eosinophils (EOS) in the nasal mucosa, and to observe allergen-induced nasal runny nose symptoms. RESULTS: Exposure to allergen caused disorganization of cilia in nasal mucosa of rats. Some cilia adhering to clumps in different directions, epithelial cilia and most villus shedding and other ultrastructural changes, EOS infiltration first increased and then decreased. No significant changes in the number of nasal grasping nasal discharge runny nose symptoms initially increased, with the prolonged exposure to allergens appeared nasal discharge obstacles. CONCLUSION: Nasal mucosal cilia are progressively damaged in AR rats induced by prolonged allergen. In addition, nasal symptoms under long-term allergen challenge may be related to the progressive damage of nasal mucosa.