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目的建立葛花降糖丸(川芎、黄连、黄芪等)的质量标准。方法采用TLC法鉴别处方中的川芎、黄连;用HPLC-ELSD法测定黄芪中黄芪甲苷的含量,色谱柱:菲罗门Luna C18(250 mm×4.6 mm,5μm),流动相:乙腈-水(36∶64);流速:1.0 m L/min;漂移管温度:105℃;载气流速:2.7 L/min。结果 TLC鉴别色谱特征斑点明显,黄芪甲苷在0.493~2.465μg范围内呈线性关系,r=0.9997;平均回收率为98.7%(n=6),RSD=1.5%。结论所建立的TLC和HPLC-ELSD方法操作简便,结果准确,重复性好,可用于葛花降糖丸的质量控制。
Objective To establish the quality standard of Gehua Jiangtangwan (Chuanxiong, Coptidis, Astragalus, etc.). Methods Chuanxiong and Coptidis Rhizoma in the prescription were identified by TLC. The content of Astragaloside IV in Radix Astragali was determined by HPLC-ELSD. The mobile phase consisted of Phenolmon Luna C18 (250 mm × 4.6 mm, 5 μm) and acetonitrile-water (36:64); flow rate: 1.0 m L / min; drift tube temperature: 105 ° C; carrier gas flow rate: 2.7 L / min. Results The spots of TLC identification chromatogram were obvious. Astragaloside had a linear relationship in the range of 0.493-2.465 μg (r = 0.9997). The average recovery was 98.7% (n = 6) and RSD was 1.5%. Conclusion The established TLC and HPLC-ELSD methods are simple, accurate, reproducible and can be used for the quality control of Ge Hua Jiang Tang Pills.