论文部分内容阅读
在介绍HCV抗体检测的血清学方法6年后,检测技术已经有了十分明显的进步。第三代ELISA试剂含有4～5个结构或非结构蛋白的片段,提高了抗体检测的敏感性和特异性。已经建立了几种检测非特异性反应的验证实验方法。急性或慢性HCV感染时的病毒血症可用RT-PCR检测,然而,对系列血清PCR结果的可靠性研究表明,十分迫切要求PCR技术的规范化。
Six years after the introduction of serological methods for the detection of HCV antibodies, there has been a marked improvement in detection technology. Third-generation ELISA reagents contain 4 to 5 structural or nonstructural protein fragments that increase the sensitivity and specificity of antibody detection. Several validated experimental methods for detecting nonspecific reactions have been established. However, viremia at acute or chronic HCV infection can be detected by RT-PCR. However, studies on the reliability of serial serum PCR results indicate that there is an urgent need to standardize PCR techniques.