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目的:观察扶正抗病毒方对A型流感病毒感染小鼠肺部病理变化与超微结构的影响。方法:采用鼻腔接种法建立A型流感病毒小鼠肺部感染模型。实验分为正常组、模型组、利巴韦林组和扶正抗病毒方组,经灌胃给药或给0.9%氯化钠溶液7d后,处理动物,常规检测肺重量指数;光学显微镜技术观察肺病理变化,透射电子显微镜技术观察肺组织超微结构。结果:①与正常组比较,模型组肺指数明显升高(P<0.01),扶正抗病毒方组肺指数降低,与模型组比较,差异显著(P<0.05)。②与正常组比较,模型组肺脏充血、水胀,呈现暗褐色外观,肺泡、细支气管等正常组织结构消失,肺泡内有大量炎症细胞浸润。扶正抗病毒方组肺组织病理变化显著改善。③与正常组比较,模型组小鼠肺组织超微结构受到破坏,核膜与质膜模糊不清,细胞器减少,线粒体嵴减少或消失,内质网出现空泡样病变,胞浆内有大量病毒颗粒。扶正抗病毒方组肺组织细胞结构较完整,细胞器丰富,线粒体和内质网的改变程度低于模型组。结论:扶正抗病毒方对A型流感病毒感染所致的肺部病理变化与超微结构有改善作用。
Objective: To observe the effect of Fuzheng Antiviral Recipe on lung pathological changes and ultrastructure in mice infected with influenza A virus. Methods: Nasal inoculation method was used to establish the lung infection model of influenza A virus in mice. The experiment was divided into normal group, model group, ribavirin group and Fuzheng antiviral group. After intragastric administration or 0.9% sodium chloride solution for 7 days, the animals were treated and the lung weight index was measured routinely. The results of light microscopy Lung pathological changes, transmission electron microscopy observation of lung tissue ultrastructure. Results: ①Compared with the normal group, the lung index of the model group was significantly increased (P <0.01); the index of the lung of the Fuzheng antiviral treatment group was lower than that of the model group (P <0.05). Compared with the normal group, the lungs of the model group were hyperemic and water-swollen with dark brown appearance. The normal tissues such as the alveoli and bronchioles disappeared, and the inflammatory cells infiltrated in the alveoli. Fuzheng antiviral group of lung tissue pathological changes significantly improved. ③ Compared with the normal group, the ultrastructure of the lung tissue in the model group was damaged, the nuclear membrane and plasma membrane were unclear, the organelles were reduced, the mitochondrial cristae were reduced or disappeared, the vacuole-like pathological changes appeared in the endoplasmic reticulum, Virus particles. Fuzheng antiviral group lung tissue cells more complete cell structure, rich organelles, mitochondria and endoplasmic reticulum changes were lower than the model group. Conclusion: Fuzheng Antiviral can ameliorate pulmonary pathological changes and ultrastructure induced by influenza A virus infection.