论文部分内容阅读
考虑到肿瘤相关抗原存在于癌细胞的表面,因而我们试图从分离人体大肠癌细胞的质膜着手,以便研究大肠癌的免疫学诊断问题。为此研究和建立了快速分离纯化大肠癌细胞质膜的方法。取外科摘除的结直肠腺癌组织,经大量生理盐水漂洗并剪成小块后,用组织捣碎机机械捣碎组织(连接调压变压器起动80V旋转3分钟)。也有用0.25％胰酶消化的,按1克组织
Considering that tumor-associated antigens are present on the surface of cancer cells, we have attempted to isolate the plasma membrane of human colorectal cancer cells in order to study the immunological diagnosis of colorectal cancer. To this end, a method for the rapid isolation and purification of the plasma membrane of colorectal cancer cells was developed and established. Surgical removal of colorectal adenocarcinoma tissue was performed after rinsing with a large amount of saline and cutting into small pieces. The tissue was minced using a tissue crusher (connected to a voltage regulator transformer to start 80V rotation for 3 minutes). Also useful with 0.25% trypsin digestion, 1 g tissue