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目的 :探讨食物纤维成分之一丁酸钠对人大肠癌细胞生长、分化的影响。方法 :应用 MTT、流式细胞仪、光镜及电镜技术 ,观察经丁酸钠诱导后低分化人大肠癌细胞系 Clone- A细胞生长及形态学变化。结果 :经丁酸钠诱导后 ,Clone- A细胞生长受到明显抑制 ;G0 / G1 期细胞百分比增加 ,S期细胞百分比减少 ,细胞增殖指数下降 ,诱导后 Clone- A细胞变长 ,出现胞浆突起 ,由圆形、不规则形变为扁平的成纤维细胞样形态。同时 ,丁酸钠作用 2 d后 ,细胞表面伸出丝状伪足 ,4d后明显变长。胞浆内质网、高尔基体数量及密度增加 ,内质网聚集核周围并铺展于胞质内 ,形态趋于正常。结论 :丁酸钠能够抑制低分化人大肠癌细胞生长 ,诱导其成熟分化 ,使其一些恶性表型逆转。
Objective: To investigate the effect of sodium butyrate, a component of dietary fiber, on the growth and differentiation of human colorectal cancer cells. METHODS: MTT, flow cytometry, light microscopy, and electron microscopy were used to observe the growth and morphological changes of Clone-A cells, a poorly differentiated colorectal cancer cell line induced by sodium butyrate. RESULTS: After sodium butyrate induction, the growth of Clone-A cells was significantly inhibited; the percentage of cells in G0/G1 phase increased, the percentage of cells in S phase decreased, and the cell proliferation index decreased. After induction, Clone-A cells became longer and cytoplasmic processes appeared. From a round, irregular shape to a flat fibroblast-like morphology. At the same time, after 2 days of sodium butyrate exposure, the fibropodia appeared on the surface of the cell and became longer after 4 days. The number and density of the cytoplasmic endoplasmic reticulum, Golgi apparatus increase, the endoplasmic reticulum aggregates around the nucleus and spreads within the cytoplasm, and the morphology tends to be normal. Conclusion: Sodium butyrate can inhibit the growth of poorly differentiated human colorectal cancer cells, induce its maturation and differentiation, and reverse some of its malignant phenotypes.