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1988—1989年在哈尔滨市郊、伊春、大庆、绥化等地的保护地黄瓜植株上采集具有花叶、疱斑、皱缩和矮化等症状的病害病标样174份。经生物测定、蚜虫传播、体外稳定性测定和血清学试验,黑龙江省黄瓜病毒病的毒原有黄瓜花叶病毒(CMV)和南瓜花叶病毒(SqMV)。CMV的病样率为36.2%,SqMV为3.48%。通过生物测定从63个CMV分离物中选出10个CMV代表分离物。经蚕豆三次单斑分离后,将10个CMV分离物接种于5科14种指示植物上,根据它们在上述指示植物上的症状反应、蚜虫传播、体外稳定性和血清学等试验的结果,我们发现黑
In 1988-1989, 174 samples of cucumber disease with mosaic, blister, shrinkage and dwarf symptoms were harvested from cucumber plants in the protected areas of Harbin, Yichun, Daqing and Suihua. The cucumber mosaic virus (CMV) and pumpkin mosaic virus (SqMV) of cucumber virus in Heilongjiang Province were determined by bioassay, aphid transmission, in vitro stability assay and serological test. The disease-like rate of CMV was 36.2% and the SqMV was 3.48%. Ten CMV representative isolates were selected from 63 CMV isolates by bioassay. After three single spot isolations from Vicia faba, 10 CMV isolates were inoculated on 14 indica plants in 5 families. Based on their results on the above-indicated plant symptoms, aphid spread, in vitro stability and serology, we Found black