目的探讨血小板膜糖蛋白Ⅱb/Ⅲa受体拮抗剂替罗非班在体内持续应用对动脉硬化兔血浆炎症因子的影响,为动脉硬化时合理应用血小板膜糖蛋白Ⅱb/Ⅲa受体拮抗剂提供理论依据。方法高脂饲料喂养新西兰大白兔12周形成动脉硬化模型后,分为4组,每组8只,分别恒速静脉滴注生理盐水、3.125、12.5 mg/L和50mg/L的替罗非班注射液,于注射0、12、24 h和48 h时,采用比浊法检测血小板最大聚集率和酶联免疫吸附法检测血浆P选择素、白细胞介素6、白细胞介素1β、肿瘤坏死因子α等炎症因子的水平,比较相关指标的变化。结果实验12、24 h和48 h时,随着替罗非班用药剂量增加,血小板最大聚集率逐渐减低(PANOVA<0.05),P选择素、白细胞介素1β、白细胞介素6、肿瘤坏死因子α逐渐减低(PANOVA<0.05)。对照组和3.125 mg/L替罗非班组12、24 h和48 h的血小板最大聚集率和0 h比较无改变(P>0.05);≥12.5 mg/L替罗非班用药后的血小板最大聚集率较用药0 h明显减低(P<0.05),P选择素、白细胞介素1β、白细胞介素6、肿瘤坏死因子α较0 h明显减低(P<0.05)。结论小剂量替罗非班并不改变血小板聚集,中剂量至大剂量静脉滴注时,血小板聚集和血清炎症因子明显抑制。 Objective To investigate the effect of continuous application of platelet glycoprotein Ⅱb / Ⅲa receptor antagonist tirofiban on the inflammatory factors in rabbits with arteriosclerosis, and to provide theory for the rational use of platelet glycoprotein Ⅱb / Ⅲa receptor antagonists in arteriosclerosis in accordance with. Methods New Zealand white rabbits were fed with high-fat diet for 12 weeks to establish the model of atherosclerosis. The models were divided into 4 groups with 8 rats in each group. The animals were given saline, 3.125,12.5 mg / L and 50 mg / L tirofiban respectively. Injection, at 0, 12, 24 h and 48 h after injection, the maximum aggregation rate of platelets was detected by turbidimetric method and the levels of plasma P-selectin, interleukin-6, interleukin-1β, tumor necrosis factor α and other inflammatory cytokines, comparing the changes of related indicators. Results At 12, 24, and 48 hours, the maximum platelet aggregation rate (P <0.05), P-selectin, interleukin 1β, interleukin 6, tumor necrosis factor α decreased gradually (PANOVA <0.05). The maximum aggregation rate of platelets in control group and tirofiban at 3.125 mg / L for 12, 24, and 48 h did not change (P> 0.05), and the maximum aggregation of platelets after treatment with ≥ 12.5 mg / L tirofiban (P <0.05). P selectin, interleukin 1β, interleukin 6 and tumor necrosis factor α were significantly lower than those at 0 h (P <0.05). Conclusion Low dose of tirofiban does not change platelet aggregation. Platelet aggregation and serum inflammatory cytokines were significantly inhibited in medium dose to high dose intravenous drip.