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以猴艾滋病病毒SIV作为人艾滋病病毒HIV体外模型,观察了兴安升麻皂甙(Cd-S)在Hut-78-SIV体外培养系统对SIV的抑制作用,并与阳性药叠氮胸苷(AZT)的作用进行了比较。结果200mg/mlCd-S可抑制荧光阳性细胞数。抑制率为24.00%,SIV产量下降2~3个单位,细胞病变程度亦有所缓解。与阳性药AZT相比,Cd-S对SIV的抑制作用仅为轻度。为探讨其作用机制,同时观察了Cd-S在体外PHA刺激的淋巴细胞培养系统对3H-TdR转运的影响,结果表明175.0μg/ml剂量Cd-S可明显抑制3H-TdR的转运,抑制率达93.85%,提示Cd-S是通过抑制细胞膜的核苷转运过程,导致SIV在宿主细胞内自身DNA合成受限,SIV产量下降,表现为Cd-S对SIV的抑制作用。
Using monkey AIDS virus SIV as human HIV HIV in vitro model, we observed the inhibition of SIV by Huh-78-SIV in vitro culture system and the positive drug zidovudine (AZT). The role was compared. Results 200 mg/ml Cd-S inhibited the number of positive cells. The inhibition rate was 24.00%, SIV production decreased by 2 to 3 units, and the extent of cytopathy also eased. Compared with the positive drug AZT, the inhibitory effect of Cd-S on SIV was only mild. To investigate its mechanism of action, the effect of Cd-S on the 3H-TdR transport in PHA-stimulated lymphocyte culture system was also observed. The results showed that the dose of 175.0 μg/ml Cd-S could significantly inhibit the 3H-TdR transport and inhibit The rate reached 93.85%, suggesting that Cd-S inhibits the nucleoside transport process of the cell membrane, resulting in the limited synthesis of SIV in the host cell’s own DNA, and a decrease in the production of SIV, manifested as the inhibitory effect of Cd-S on SIV.