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目的了解新建立免疫吸附(ELISA)法对人血清沙眼衣原体感染检测的敏感性和特异性。方法收集2014年3月-12月天津医科大学总医院非性病门诊15~40岁就诊者血清共300例。采用酶联免疫吸附(ELISA)法检测其血清中抗衣原体质粒编码蛋白3(Pgp3)、CT875编码蛋白(CT875)、CT694编码蛋白(CT694)的抗体。同时采用微量免疫荧光(MIF)法对血清样本进行检测。比较两种方法的检测结果。结果 ELISA法共筛选出三种免疫优势蛋白抗体阳性者63例,其中MIF法证实阳性者62例。300例就诊者血清中三种免疫优势蛋白抗体的总检出率为21.00%。以MIF法为标准,新建立ELISA法的灵敏度为95.38%,特异度为99.57%。结论新建立的ELISA方法具有较高的敏感度与特异度,适合用于沙眼衣原体感染的初筛。
Objective To understand the sensitivity and specificity of the newly established immunosorbent assay (ELISA) for detection of human serum Chlamydia trachomatis infection. Methods A total of 300 serum samples from 15 to 40 years old from non-STD clinics of Tianjin Medical University General Hospital from March 2014 to December 2014 were collected. Serum anti-Chlamydia pneumoniae antibody encoding Pgp3, CT875 (CT875) and CT694 (CT694) were tested by enzyme linked immunosorbent assay (ELISA). Serum samples were also detected by micro-immunofluorescence (MIF). Compare the results of the two methods. Results 63 cases of three immunodominant protein antibodies were screened by ELISA, of which 62 cases were positive by MIF assay. The total detection rate of three immunodominant protein antibodies in sera of 300 patients was 21.00%. The MIF method as the standard, the newly established ELISA sensitivity was 95.38%, specificity was 99.57%. Conclusion The newly established ELISA method has high sensitivity and specificity and is suitable for primary screening of Chlamydia trachomatis infection.