论文部分内容阅读
FRTL细胞分别在含有10-6-10-3mol／L碘化钾的培养基中培养24、48和72 h,采用RT- PCR和Western印迹,检测钠碘转运体(NIS)mRNA和蛋白水平的变化。FRTL细胞在10-6-10-3mol／L高碘培养基中培养24、48 h,NIS mRNA水平与对照组相比。差异无统计学意义。而FRTL细胞在10-6-10-3 mol／L高碘培养基中培养48、72 h,NIS蛋白呈逐渐下降趋势,碘浓度越高,NIS蛋白下降越明显。本研究显示急性碘过量不影响NIS mRNA的表达,但可下调NIS蛋白,碘过量通过转录后水平调节NIS的基因表达。
FRTL cells were cultured in medium containing 10-6-10-3mol / L potassium iodide for 24,48 and 72 h, respectively. The mRNA and protein levels of sodium iodide transporter (NIS) were detected by RT-PCR and Western blotting. FRTL cells were cultured in 10-6-10-3mol / L high iodine medium for 24,48 h, and the NIS mRNA level was compared with the control group. The difference was not statistically significant. While FRTL cells cultured in high iodine medium of 10-6-10-3 mol / L for 48 and 72 h, the NIS protein showed a gradual downward trend. The higher the iodine concentration, the more obvious the decline of NIS protein. This study shows that acute iodine excess does not affect the expression of NIS mRNA, but can down-regulate NIS protein. Iodine excess regulates NIS gene expression through post-transcriptional level.