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羟基红花黄色素A是中药红花的主要活性成分,具有活血通经、散瘀止痛的功效,同时对血瘀风动帕金森病也有着确切的治疗效果,但当前研究多集中于单一的信号通路,不利于药物的临床转化和推广.本研究采用鱼藤酮构建PD细胞模型,通过细胞活力和线粒体膜电位评价HSYA对PD细胞模型的保护作用.利用TTD数据库查询PD相关治疗靶点,Swiss Target Prediction数据库查询HSYA相关靶点,STRING数据库查询共同靶点的基因相互作用关系,ClueGO对共同靶点及其相互作用靶点进行通路富集分析,探索综合干预机制.结果表明,鱼藤酮能成功构建PD细胞模型,HSYA对PD细胞模型有明显的保护作用.通过网络药理学分析,查询到PD相关治疗靶点36个,HYSA相关靶点88个.PD和HSYA的共同靶标为FKBP1A、HTR1A、SLC6A4和SLC6A3,通过REACTOME对4个共同靶标及其相互作用靶标进行富集分析,得到8个细胞信号通路,通过生物过程途径富集分析得到6个细胞生物过程.“,”As an effective component of safflower,hydroxysafflor yellow A(HSYA)has the effect of promoting blood circulation,removing blood stasis,and relieving pain,and it has a certain effect on blood stasis and wind-induced Parkinson’s disease(PD).However,the current research mostly involves a single intervention mechanism,which is not conducive to the clinical transformation of this type of drugs.In the present study,rotenone was used to construct a PD cell model,and the protective effect of HSYA on the PD cell model was evaluated by cell viability and mitochondrial membrane potential.TTD database was used to query PD-related therapeutic targets,Swiss Target Prediction database to query HSYA-related targets,STRING database was used to search the gene interaction relationship of common targets,and ClueGO pathway was adopted to enrich and analyze common targets and their interaction targets,as well as to explore the comprehensive intervention mechanism.The results showed that rotenone could successfully establish the PD cell model,and HSYA had significant protective effect on PD cell model.Through the network pharmacological analysis,36 PD-related therapeutic targets and 88 HSYA-related targets were queried.The common targets of PD and HSYA were FKBP1A,HTR1A,SLC6A4 and SLC6A3.To enrich four common targets and their interaction targets through REACTOME pathway,eight cell signal pathways were obtained,and six cell biological processes were obtained through biological process pathway enrichment.